P2 (5 -TTAAGCTTATACGGGACATA GTGCACAGCC) and N3-P1 (5 -AAGGATCCTGAACCGCTCTC TCTTCCTTCC). N3-P1 primes
P2 (five -TTAAGCTTATACGGGACATA GTGCACAGCC) and N3-P1 (5 -AAGGATCCTGAACCGCTCTC TCTTCCTTCC). N3-P1 primes promptly upstream of the ATG translation start out codon of NIMIN3. The resulting 1.4 kb fragment was ligated to…